The chloroform, methanol and aqueous extracts of Anogeissus leiocarpus fruits were tested for antimicrobial activity against one Gram positive bacteria (Staphyllococcus aureus ) and four Gram negative bacteria (Escherichia coli,Klebsiela pnuemoniae,Proteus vulgaris and Pseudomonas aeruginosa) and against three fungi (Aspergillus niger,Candida albicans and Sacchromyces cervisiae) in comparison to two reference antibiotics(Ampicillin and Gentamicin) and two reference antifungal drugs(Clotrimazole and Nystatin) using the cup plate agar diffusion method.
The fruit methanol extract of Anogeissus leiocarpus showed the highest antimicrobial activity followed by the aqueous extract of the plant and the lowest antimicrobial activity was reported for the chloroform extract.
The active methanol and aqueous extracts of A. leiocarpus were further tested against 30 clinical isolates, isolated from different sources from Soba Hospital, Khartoum Teaching Hospital Laboratory and the National Laboratory. Their minimum inhibitory concentrations were also determined.
Reference antibiotics and antifungal drugs were tested against standard organisms and the results were compared with the activity o the plant extracts.
Keywords: Antimicrobial, Anogeissus leiocarpus fruits, minimum inhibitory concentration, methanol and aqueous extracts.